- Indra Stefanianna Christopherson (1969–1970)
- Heather North (1970–1985, 1997, 2003)
- Kellie Martin (1988–1991)
- Mary Kay Bergman (1998–1999)
- Grey DeLisle (2001–present)
- Sarah Michelle Gellar (Scooby-Doo (2002), Scooby-Doo 2: Monsters Unleashed (2004))
- Kate Melton (Scooby-Doo! The Mystery Begins (2009), Scooby Doo! Curse of the Lake Monster (2010)
Together with her other teenage cohorts, Fred Jones, Shaggy Rogers, Velma Dinkley, and Shaggy's pet Great Dane Scooby-Doo, Daphne would engage in solving various mysteries. Daphne was protrayed as the enthusiastic, but clumsy and danger-prone, member of the gang, who always follows her intuition. She would occasionally get kidnapped, tied up, gagged and left imprisoned. Scooby and Shaggy usually save her, but sometimes Fred and Velma do it. Very occasionally, Daphne's rescued by all of the gang. But as the franchise went on, she became a stronger, more independent character, who can take care of herself. It should be noted that Daphne's character is the most developed in the starring cast, going from a klutzy teenager to a successful journalist to an ingenuous fashionista to a black belt martial artist. When Scooby is nowhere to be found, Daphne also yells "Scooby-Doo! Where Are You?!".
Her usual appearance consists of a purple dress, pink pantyhose, purple shoes, and a green scarf. In Scooby-Doo and the Cyber Chase, she wore a purple and green three-piece suit with matching shoes. As a child, she wore a pink sweater, red skirt, and pink pantyhose with white go-go boots.
Some fans have posited that Fred and Daphne share an attraction to each other.
In the movie Scooby-Doo on Zombie Island, Daphne had a very successful investigative TV series called Coast to Coast with Daphne Blake on a fictional channel called "Americana", which the show had aired on for two seasons. The producer of the show was Fred Jones.
Jeffrey P. Dennis, author of the journal article "The Same Thing We Do Every Night: Signifying Same-Sex Desire in Television Cartoons", said that several commentators argued that Daphne and Velma were a homosexual couple, citing Saturday Morning Fever authors Kevin and Timothy Burke. Martin Goodman of Animation World Magazine responded, stating that "Any serious reading of this section in Saturday Morning Fever (pp. 105-111) quickly reveals that the Burkes were, in a most un-academic fashion, goofing."
Relatives of Daphne shown during the series' run include:
- George R. Blake: Daphne's father, the creator of the product "Blake's Bubbles". Voiced by Frank Welker.
- Elizabeth Blake: Daphne's mother. Voiced by Kath Soucie.
- Uncle Matt: Daphne's uncle, a cattle rancher.
- John Maxwell: Daphne's uncle, a movie director.
- Olivia Derby: Daphne's aunt.
- Jennifer: Daphne's cousin.
- Danica LaBlake: Daphne's cousin, a famous French model. Voiced by Vanessa Marshall.
- Shannon Blake: Daphne's Scottish cousin. Voiced by Grey DeLisle.
- Daisy Blake: Daphne's sister
- Dorothy Blake: Daphne's sister
- Delilah Blake:Daphne's sister
- Dawn Blake: Daphne's sister
Daphne, as portrayed by Sarah Michelle Gellar in Scooby Doo 2: Monsters Unleashed.Daphne was portrayed by Sarah Michelle Gellar in the film Scooby Doo and its sequel, Scooby Doo 2: Monsters Unleashed. Her husband Freddie Prinze, Jr. played Fred in the other Scooby-Doo movies also. In the movies, Daphne and Fred began a relationship in the first, that followed on through the second. Unlike the previous incarnation of the character, Gellar's version of Daphne is trained in martial arts during Mystery Inc.'s year-long departure in the first film, as she is tired of being a damsel in distress on every case that she is involved in with the gang. Although in the first movie, she is captured by the masked wrestler Zakos, but she beats him in a fight at the end.
Daphne is portrayed by Kate Melton in the 2009 live-action movie Scooby-Doo! The Mystery Begins, its sequel Scooby Doo! Curse of the Lake Monster (which is scheduled to begin filming in 2010), and two other Scooby-Doo movies.
- ^ Dennis, Jeffrey P. "The Same Thing We Do Every Night: Signifying Same-Sex Desire in Television Cartoons." Journal of Popular Film & Television. Fall 2003. Volume 31, Issue 3. 132-140. 9p, 3bw. Within the PDF document the source info is on p. 134 (3/10)
- ^ Goodman, Martin. "Deconstruction Zone — Part 2." Animation World Magazine. 2." Retrieved on October 30, 2009.